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2009 Fiscal Year Final Research Report

Structural analysis of microtubule motor using peptide array system

Research Project

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Project/Area Number 20870033
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionYokohama City University

Principal Investigator

HAYASHI Ikuko  Yokohama City University, 生命ナノシステム科学研究科, 准教授 (80464527)

Project Period (FY) 2008 – 2009
Keywords微小管 / 細胞骨格 / x線結晶構造解析 / ペプチドアレイ
Research Abstract

I have established the bacterial over-expression system of the FHA domain region in KIF1C. The oligomerization state of recombinant proteins was examined by gel-filtration and chemical cross-linking analyses. We are now investigating the intra-molecular interaction site using peptide-array system.

  • Research Products

    (5 results)

All 2009 2008 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Characterisation of a conserved "threonine clasp" in CAP-Gly domains: role of a functionally critical OH/πinteraction in protein recognition2008

    • Author(s)
      MJ Plevin, I Hayashi, M Ikura
    • Journal Title

      J Am Chem Soc 12

      Pages: 14918-14919

    • Peer Reviewed
  • [Journal Article] 微小管伸長端結合蛋白質の分子制御機構2008

    • Author(s)
      林郁子
    • Journal Title

      生化学 6

      Pages: 521-530

  • [Presentation] 微小管伸長端結合蛋白質CLASP2の微小管安定化機構の解析2009

    • Author(s)
      真木孝尚, 林郁子
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      20091200
  • [Presentation] Crystal Structure of Microtubule binding protein CLASP22009

    • Author(s)
      T Maki, I Hayashi
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      米国ニューハンプシャー州
    • Year and Date
      20090700
  • [Remarks] 横浜市立大学大学院バイオエキスパート研究体験シリーズ「イオン濃度を測る・蛍光計測」(平成22年5月29日)にて研究成果の一部を公開

    • URL

      http://www.tsurumi.yokohama-cu.ac.jp/biophys/hayashi

URL: 

Published: 2011-06-18   Modified: 2016-04-21  

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