2009 Fiscal Year Final Research Report
Structural and functional analyses on a novel mechanism of homologous recombination suppression.
| Project/Area Number |
20870042
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
FUKUI Kenji The Institute of Physical and Chemical Research, 機能解析第2研究チーム, 研究員 (00466038)
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| Project Period (FY) |
2008 – 2009
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| Keywords | DNA / 相同組換え抑制 / X線結晶構造解析 / DNA切断酵素 |
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| Research Abstract |
Recent studies revealed the existence of a novel anti-recombination enzyme, MutS2 ; however the mechanism by which MutS2 inhibits homologous recombination has been unknown. To elucidate the mechanism, we performed structural and functional analyses on Thermus thermophilus MutS2. X-ray crystallographic analysis on the C-terminal domain of MutS2 revealed that this domain resembles the structures of known endonucleases. Furthermore, biochemical experiments demonstrated that MutS2 preferentially incises the early intermediates in homologous recombination. These results indicate that MutS2 suppresses homologous recombination through a novel mechanism involving resolution of early intermediates.
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