2009 Fiscal Year Final Research Report

Genome wide localization analysis of BphT1 binding region on Rhodococcus jostii RHA1.

Research Project

Project/Area Number	20880031
Research Category	Grant-in-Aid for Young Scientists (Start-up)
Allocation Type	Single-year Grants
Research Field	Applied microbiology
Research Institution	Okayama University of Science
Principal Investigator	HARA Hirofumi Okayama University of Science, 工学部, 講師 (80511071)
Project Period (FY)	2008 – 2009
Keywords	放線菌 / 環境汚染物質 / ChIP-chip解析 / 二成分制御系 / 転写制御因子
Research Abstract	Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades polychlorinated biphenyls (PCBs). Two-component signal transduction system, BphT1T2, is necessary for activation of biphenyl degradation genes in R. jostii RHA1. In this study, we performed global chromatin immunopercipitation-on-chip (ChIP-chip) analysis to identify novel BphT1 binding region in R. jostii RHA1. As a result, we identified 9 ORFs as direct target of BphS1T1, which including IclR type transcriptional regulator, possible endonuclease, and some hypothetical proteins. This result indicated that BphS1T1 is a master regulator of biphenyl degradation, and directly activated to transcriptional regulator and endonibonuclease to regulate expression level of many ORFs in RHA1.

(2 results)