2009 Fiscal Year Final Research Report
Molecular biological analysis of the Babesia parasite transmission blocking mechanism conducted by tick aminopeptidase.
| Project/Area Number |
20880040
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
HATTA Takeshi National Agricultural Research Organization, 動物衛生研究所, 研究員 (00455304)
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| Project Period (FY) |
2008 – 2009
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| Keywords | マダニ / アミノペプチダーゼ / バベシア原虫 / マダニ媒介感染症 |
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| Research Abstract |
Tick is well known biological vector of zoonotic infectious diseases such as Lyme disease but the tick molecular function regarding pathogen transmission is poorly understood. A terminal enzyme of host blood digestion in tick midgut was an aminopeptidase, HlLAP which was shown to be involved in the regulatory effect on babesial migration in tick by inhibiting its invasion. Based on the cellular biological study, we observed that HlLAP gene silencing by RNA interference induced the immature generation of peritrophic membrane (PM) in midgut. PM is the mechanical barrier of midgut epithelium against bacterial or xenobiotic substances in host blood meal. Therefore, it was assumed that HlLAP which was responsible for the generation of PM disruption of which inhibited indirectly an invasion of Babesia parasite into the midgut epithelium.
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[Journal Article] Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes.2010
Author(s)
Hatta T, Tsuji N, Miyoshi T, Islam MK, Alim MA, Yamaji K, Anisuzzaman, Fujisaki K
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Journal Title
Parasitol Int 59(2)
Pages: 286-289
Peer Reviewed
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