Therapeutic efficacy by adoptive transfer of iNKT cells and B-1Bcells to severe acute bacterial pneumonia

2009 Fiscal Year Final Research Report

• Project/Area Number: 20890017
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Infectious disease medicine
• Research Institution: Tohoku University
• Principal Investigator: YAMAMOTO Natsuo Tohoku University, 大学病院, 助教 (50466562)
• Project Period (FY): 2008 – 2009
• Keywords: 肺炎球菌性急性肺炎 / B-1B細胞 / activation-induced cytidine deaminase (AID) / 抗ホスホリルコリンIgM抗体 / iNKT細胞 / 接触性過敏反応(Contact Sensitivity ; CS) / ELISPOTアッセイ / Interleukin(IL)-13

Research Abstract

B-1B cells were rapidly activated by an acute lung infection with a crucial involvement of iNKT cells upstream. B-1B cells then migrate to the spleen where an increase of B-1B cell numbers were shown by the measurements of ELISPOT assay for anti-phosphorylcholine IgM producing cells. Further, the activated B-1B cells acted more protectively with utilizing activation-induced cytidine deaminase (AID) within 2 days post airway infection than their naive status.

Research Products

• [Journal Article] B-1細胞と呼吸器疾患 (2010)
  • Author(s): 山本夏男, 金光敬二
  • Journal Title: 炎症と免疫 18巻(in press)
• [Presentation] Pathogen specific IL-13-producing T cells and B-1B cell interaction in lung associated lymph nodes and spleen (2009)
  • Author(s): Yamamoto N, Steven KM, Aoyagi T, Charles DC, Kunishima H, Kawakami K, Kaku M, Askenase PW, et al.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場 (大阪府)
  • Year and Date: 2009-12-02
• [Presentation] IL-13 Dependent Early Protective Role of B-1 Cell Function in Acute Bacterial Pneumonia (2009)
  • Author(s): Yamamoto N, Steven KM, Aoyagi T, Hatta M, Kunishima H, Miyazato A, Hirakata Y, Kawakami K, Kaku M, Askenase PW, et al.
  • Organizer: 第49回日本呼吸器学会学術講演会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2009-06-12
• [Presentation] 23価肺炎球菌ワクチンに対する液性免疫応答と、natural killer T細胞の役割に関する検討 (2009)
  • Author(s): 宮坂智充, 青柳哲史, 内山美寧, 國島広之, 山本夏男, 平潟洋一, 石井恵子, 大石和徳, 賀来満夫, 川上和義
  • Organizer: 第83回日本感染症学会総会学術講演会
  • Place of Presentation: 京王プラザホテル(東京都)
  • Year and Date: 2009-04-23
• [Presentation] Roles of B-1B cells and AID in early acquired resistance to acute S. pneumoniae lung infection (2008)
  • Author(s): Yamamoto N, Steven KM, Kawakami K, Kaku M, Askenase PW.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2008-12-01
• [Presentation] IL-13 Dependent Role of Early Protective B-1 Cell Function in Lung Infection with S. pneumoniae (2008)
  • Author(s): Yamamoto N, Steven KM, Kawakami K, Kaku M, Askenase PW.
  • Organizer: 第48回国際化学療法学会(ICAAC)
  • Place of Presentation: Washington Convention Center (ワシントンDC, USA)
  • Year and Date: 2008-10-28
• [Presentation] IL-13 dependent protective role of early B-1 cell function in lung infection with S. pneumoniae (2008)
  • Author(s): Yamamoto N, Kunishima H, Charles DC, Steven KM, Marian S, Kawakami K, Askenase PW, Kaku M.
  • Organizer: 第8回あわじしま感染症・免疫フォーラム
  • Place of Presentation: あわじ夢舞台国際会議場(兵庫県)
  • Year and Date: 2008-09-09
• [Presentation] B-1B細胞由来獲得型IgM抗体が急性細菌肺炎時にAID及びIL-13依存的に応答する機構の解明 (2008)
  • Author(s): 山本夏男, Steven K., 青柳哲史, 丹野大樹, 國島広之, 川上和義, 賀来満夫, Philip W. Askenase
  • Organizer: 3学会合同大会 (第73回日本インターフェロン・サイトカイン学会学術集会/第19回日本生体防御学会学術総会/第45回補体シンポジウム)
  • Place of Presentation: 北海道大学学術交流会館(北海道、札幌市)
  • Year and Date: 2008-07-11
• [Remarks] ホームページ等
  • URL: http://www.fmu.ac.jp/kenkyu/Profiles/39/0003823/profile.html