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2009 Fiscal Year Final Research Report

The Possibility of Cancer Diagnosis with Fluidity Sensitive Fluorescence Measurement

Research Project

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Project/Area Number 20890029
Research Category

Grant-in-Aid for Young Scientists (Start-up)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KANEKO Tsuyoshi  University of Tsukuba, 大学院・人間総合科学研究科, 講師 (90510181)

Project Period (FY) 2008 – 2009
Keywords蛍光偏光解消法 / 膜流動性 / 光学的生検 / 消化器内視鏡
Research Abstract

BACKGROUND AND AIMS : The cellular membrane fluidity changes in response to physiological and pathological stimuli. We showed that the indomethacin treatment increased the fluidity of gastric cellular membrane using a specially designed microscope based system to measure the fluorescence polarization of gastric mucosa-derived RGM-1 cells stained with dodecanoylaminofluorescein (DAF). In the present study, the membrane fluidity of gastric cancer specimens and their surrounding normal mucosa were investigated whether carcinogenesis increased the cellular membrane fluidity. PATIENTS AND METHODS : Gastric cancer specimens from a total of 11 gastric cancer patients that were resectioned with Endoscopic Submucosal Dissection procedure were investigated. After washed with phosphate buffered saline, the specimens were topically treated with DAF and fluorescence polarization (FP) values on the surface cellular membrane were measured and calculated with an image intensified CCD-mounted fluorescence microscope and an image-processor. Diagnostic accuracy was evaluated and compared with pathological sections stained by H&E stain. RESULTS : The FP values of histologically-confirmed human astric cancer showed significantly smaller than those of surrounding normal gastric mucosa, indicating that cellular membrane fluidity was decreased in human cancer tissues. CONCLUSION : The present study revealed the cellular membrane fluidity increased during gastric carcinogenesis. The results exhibited the possibility of gastric cancer diagnosis with fluidity measurement.

  • Research Products

    (5 results)

All 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results)

  • [Journal Article] ALAを用いた胃癌の光線力学的診断2009

    • Author(s)
      松井裕史, 金子剛
    • Journal Title

      臨床消化器内科 24巻

      Pages: 1379-1386

  • [Journal Article] Neoplastic transformation and induction of H^+,K^+-adenosine triphosphatase by N-methyl-N'-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line2008

    • Author(s)
      O. Shimokawa, H. Matsui, Y. Nagano, T. Kaneko, T.
    • Journal Title

      In Vitro Cellular & Developmental Biology 44(1-2)

      Pages: 26-30

    • Peer Reviewed
  • [Presentation] 米国消化器病週間 (DDW2009)2009

    • Author(s)
      Kaneko T
    • Organizer
      The fluorescence assessment of cellular membrane fluidity in human gastric cancer specimens
    • Place of Presentation
      Chicago USA
    • Year and Date
      2009-06-03
  • [Presentation] 蛍光偏光解消法によるNSAIDs起因性胃粘膜細胞傷害の捕捉2009

    • Author(s)
      金子剛, ほか
    • Organizer
      第27回サイトプロテクション研究会
    • Place of Presentation
      京都
    • Year and Date
      2009-03-27
  • [Presentation] 米国消化器病週間 (DDW2008)2008

    • Author(s)
      Shimokawa O, Kaneko T
    • Organizer
      Malignant Transformation of Rat Gastric Epithelial RGM-1 Cell Line Induced By Carcinogen N-methyl-N-nitro-N-Nitroso -guanidine treatment Was Accompanied with the Ectopic Expression of H^+, K^+ Adenosine Triphosphatase
    • Place of Presentation
      San Diego USA
    • Year and Date
      2008-05-21

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Published: 2011-06-18   Modified: 2016-04-21  

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