2009 Fiscal Year Final Research Report
Establishment of a mouse lung-specific gene suppression system using adeno-associated virus carrying short hairpin RNA
Project/Area Number |
20890052
|
Research Category |
Grant-in-Aid for Young Scientists (Start-up)
|
Allocation Type | Single-year Grants |
Research Field |
Respiratory organ internal medicine
|
Research Institution | The University of Tokyo |
Principal Investigator |
KAGE Hidenori The University of Tokyo, 医学部附属病院, 助教 (80513390)
|
Project Period (FY) |
2008 – 2009
|
Keywords | アデノ随伴ウイルス / short hairpin RNA / RNA干渉 / びまん性肺疾患 / Tgfβ1 |
Research Abstract |
We demonstrate that the AAV type 2/5 hybrid vector can carry short hairpin RNA, transduce mouse alveolar epithelium-derived LA-4 cells, and suppress Tgfβ1 expression. This study opens the possibility to suppress an arbitrary gene function in lung alveolar epithelium-derived cells, and help analyze the pathogenesis of pulmonary diseases.
|
-
[Presentation] Delivery of short interfering RNA using adenoassociated virus type 2/52010
Author(s)
Kage H, Sunohara M, Kawakami M, Emoto N, Watanabe K, Aki N, Hamano E, Ohishi N, Nagase T, Takai D
Organizer
American Thoracic Society International Conference
Place of Presentation
New Orleans, CA
Year and Date
20100514-20100519
-
[Presentation] Delivery of short interfering RNA to alveolar epithelium-derived cells using adeno-associated virus type 2/52010
Author(s)
Kage H, Sunohara M, Kawakami M, Kitano K, Emote N, Watanabe K, Aki N, Kusakabe M, Hamano E, Ohishi N, Nagase T, Takai D
Organizer
AACR-IASLC Joint Conference on Molecular Origins of Lung Cancer
Place of Presentation
Coronado, CA
Year and Date
20100111-20100114
-
[Presentation] Knockdown of Tgfβ1 in alveolar epithelium-derived LA-4 cells using replication-defective adeno-associated virus carrying short hairpin RNA2009
Author(s)
Kage H, Aki N, Emote N, Watanabe K, Kutomi T, Kusakabe M, Hamano E, Kitagawa H, Ohishi N, Nagase T, Yatomi Y, Takai D
Organizer
American Thoracic Society International Conference
Place of Presentation
San Diego, CA
Year and Date
20090515-20090520
-
[Presentation] Knockdown of Tgfβ1 in alveolar epithelium-derived LA-4 cells using replication-defective adeno-associated virus carrying short hairpin RNA
Author(s)
Kage H, Aki N, Emote N, Watanabe K, Kutomi T, Kusakabe M, Hamano E, Kitagawa H, Ohishi N, Nagase T, Yatomi Y, Takai D
Organizer
第49回日本呼吸器学会
Place of Presentation
東京