2009 Fiscal Year Final Research Report
Analysis of RNA editing enzyme abnormality in the spinal motor neurons of sporadic amyotrophic lateral sclerosis
| Project/Area Number |
20890054
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIDEYAMA Takuto The University of Tokyo, 保健・健康推進本部, 助教 (30511456)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 筋萎縮性側索硬化症(ALS) / RNA編集異常 / 剖検脳脊髄組織 / GluR2Q / R部位 / ADAR2 |
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| Research Abstract |
In humans, all GluR2 mRNAs in neurons are completely edited at the Q/R site and the majority of AMPA receptors have GluR2 in their composition, making AMPA receptors Ca^<2+>-impermeable. RNA editing at this site is specifically catalyzed by adenosine deaminase acting on RNA 2 (ADAR2) in mammals including humans. We analyzed the expression levels of ADAR2 mRNA and its activity at the GluR2 Q/R site on laser-captured motor neurons from 29 cases with sporadic ALS compared with those from normal subjects. We demonstrate that inefficient GluR2 Q/R site-editing occurs in a significant proportion of motor neurons in all the ALS cases examined. Universal reduction of ADAR2 in motor neurons is likely a pathognomonic molecular change in sporadic ALS.
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[Presentation] RNA editing enzyme abnormality in sporadic amyotrophic lateral sclerosis2009
Author(s)
Hideyama T, Yamashita T, Suzuki T, Kimura D, Tsuji S, Higuchi M, Peter H, Seeburg, Takahashi R, Misawa H, Kwak S
Organizer
第82回日本薬理学会 (招待口演)
Place of Presentation
横浜
Year and Date
2009-03-18
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[Presentation]2008
Author(s)
Hideyama T, Yamashita T, Tsuji S, Misawa H, Takahasi R, Suzuki T, Kwak S
Organizer
Slow neuronal death of motor neurons in sporadic ALS mouse model by RNA editing enzyme ADAR2 knockout mice
Place of Presentation
Washington. D.C (USA)
Year and Date
2008-11-19
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