Investigation of a new treatment for implantation failure patients using peripheral monocytes

2009 Fiscal Year Final Research Report

• Project/Area Number: 20890120
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: NAKAMURA Hitomi Osaka University, 医学部附属病院, 特任臨床検査技師 (80467571)
• Project Period (FY): 2008 – 2009
• Keywords: 不妊症 / 着床 / 子宮 / マクロファージ

Research Abstract

The uterine endometrium undergoes dramatic structural and functional changes to allow the embryo to attach and implant in early pregnancy. In mice, macrophages are recruited into the endometrium by exposure to seminal plasma and these cells appear able to influence local cell communication and tissue remodeling events to promote uterine receptivity for embryo implantation. Our previous study showed that U937 macrophages regulate embryo adhesion molecule expression in human endometrial cells via LIF-mediated STAT3 signalling. In this study, we utilized an in vitro model comprising peripheral blood monocytes (PBMC) and Ishikawa cells to further explore regulation of macrophage-uterine epithelial cell communication. The signaling characteristics of PBMCs are changed by exposure to ovarian steroid hormones in the menstrual cycle, as well as by male seminal plasma, and that these factors influence PBMC capacity to regulate uterine epithelial cell adhesion molecule expression.

Research Products

• [Journal Article] Temporal and spatial expression of tumor-associated antigen RCAS1 in pregnant mouse uterus. (2010)
  • Author(s): Tskitishvili, E., Nakamura, H., Kinugasa-Taniguchi, Y., Kanagawa, T., Kimura, T., Tomimatsu, T., Shimoya, K.
  • Journal Title: Am J Reprod Immunol 63(2) Pages: 137-143
  • Peer Reviewed
• [Journal Article] Two Multipotential Transcription Factors, NE-kappaB and STAT3, play Critical and Hierarchal Roles for Implantation. (2010)
  • Author(s): Kimura T, Ogita K, Kumasawa K, Koyama S, Tsutsui T, Nakamura H
  • Journal Title: Indian J Physiol Pharmacol. 54(5) Pages: 27-32
  • Peer Reviewed
• [Journal Article] 妊娠子宮局所におけるマクロファージの役割 (2010)
  • Author(s): 中村仁美, 木村正
  • Journal Title: 医学のあゆみ 233(2) Pages: 141-145
  • Peer Reviewed
• [Journal Article] Calcineurin/NFAT pathway: a novel regulator of parturition. (2009)
  • Author(s): Tabata, C., Ogita, K., Sato, K., Nakamura, H., Qing, Z., Negoro, H., Kumasawa, K., Temma-Asano, K., Tsutsui, T., Nishimori, K., Kimura, T.
  • Journal Title: Am J Reprod Immunol 62(1) Pages: 44-50
  • Peer Reviewed
• [Journal Article] 着床期子宮局所におけるSTAT-3活性の役割 (2009)
  • Author(s): 中村仁美, 香山晋輔, 谷口武, 荻田和秀, 熊澤恵一, 張慶, 筒井建紀, 古山将康, 杉野法広, 田村博史, 金田安史, 木村正
  • Journal Title: 生殖内分泌学会雑誌 14 Pages: 35-40
  • Peer Reviewed
• [Journal Article] 着床不全の原因検索とそれに関連する遺伝子に関する話題-一過性遺伝子導入法を用いた着床不全マウスでの検討- (2008)
  • Author(s): 筒井建紀, 中村仁美, 荻田和秀, 香山晋輔, 古山将康, 木村正
  • Journal Title: 産婦人科の実際 57(2) Pages: 217-226
  • Peer Reviewed
• [Presentation] Progesterone and estrogen receptors regulate the STAT3 activity in uterus during implantation. (2010)
  • Author(s): Nguyen TM, Nakamura H, Tsutsui T, Kimura T
  • Organizer: 第135回日本生殖医学関西支部集談会、第40回関西アンドロロジーカンファレンス
  • Place of Presentation: 大阪市立大学医学部
  • Year and Date: 2010-03-13
• [Presentation] Bio-nanocapsule(BNC)を用いた子宮に対するdrug delivery systemの開発 (2009)
  • Author(s): 根來英典, 中村仁美, 松崎高志, 黒田俊一, 熊澤恵一, 筒井建紀, 木村正
  • Organizer: 第24回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2009-11-28
• [Presentation] 子宮の着床機能を評価するための生理的パラメーターの検索 (2009)
  • Author(s): 中村仁美, Nguyen TM, 細野剛良, 木村正
  • Organizer: 第24回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2009-11-27
• [Presentation] 着床期子宮局所におけるマクロファージの再構築 (2009)
  • Author(s): 宇津木裕貴, 中村仁美, Jasper MJ, Robertson SA, 香山晋輔, 田畑知沙, 筒井建紀, 木村正
  • Organizer: 第24回日本生殖免疫学会総会・学術集会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2009-11-27
• [Presentation] 分子標的治療をめざしたin-vivo gene transfer systemを用いた着床不全の病態の解析 (2009)
  • Author(s): 中村仁美
  • Organizer: 第27回日本受精着床学会総会・学術講演会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-08-07
• [Presentation] Investigation of uterine parameters to evaluate the prospects of uterine receptivity. (2009)
  • Author(s): Nakamura H, Hosono T, Minato K, Nguyen TM, Kimura T
  • Organizer: IUPS Satellite Symposium on Endometrial Receptivity and Blastocyst Implantation
  • Place of Presentation: Inamori Hall, Kyoto University
  • Year and Date: 2009-07-25
• [Presentation] Endometrial STAT-3 activation and implantation failure. (2009)
  • Author(s): Kimura T, Nakamura H, Tsutsui T
  • Organizer: IUPS Satellite Symposium on Endometrial Receptivity and Blastocyst Implantation
  • Place of Presentation: Inamori Hall, Kyoto University
  • Year and Date: 2009-07-25
• [Presentation] Macrophage regulation of embryo adhesion molecule expression in human endometrial cells. (2009)
  • Author(s): Nakamura H, Jasper MJ, Aplin JD, Kimura T, Robertson SA
  • Organizer: 5th International Conference on the Female Reproductive Tact Frauenchiemsee
  • Place of Presentation: Germany
  • Year and Date: 2009-05-17
• [Presentation] Mouse model of human infertility: Transient and local inhibition of endometrial STAT3 activation results in implantation failure. (2009)
  • Author(s): Kimura T, Nakamura H, Tsutsui T
  • Organizer: 5th International Conference on the Female Reproductive Tact Frauenchiemsee
  • Place of Presentation: Germany
  • Year and Date: 2009-05-17
• [Presentation] マクロファージによる着床期子宮内膜における胚接着分子の制御 (2009)
  • Author(s): 中村仁美, Jasper MJ, Aplin JD, 香山晋輔, 荻田和秀, 熊澤恵一, 筒井建紀, 木村正, Robertson SA
  • Organizer: 第61回日本産婦人科学会学術講演会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-04-04
• [Presentation] 宮局所における一過性遺伝子導入方法を用いたヒト不妊症のマウスモデルの作製 (2008)
  • Author(s): 中村仁美, 香山晋輔, 荻田和秀, 谷口武, 筒井建紀, 金田安史, 木村正子
  • Organizer: 第24回日本DDS学会
  • Place of Presentation: 六本木アカデミーヒルズ40
  • Year and Date: 20080629-20080630
• [Presentation] 一過性遺伝子導入方法を用いた着床現象の検討 (2008)
  • Author(s): 中村仁美, 香山晋輔, 谷口武, 荻田和秀, 熊澤恵一, 張慶, 筒井建紀, 古山将康, 杉野法広, 田村博史, 金田安史, 木村正
  • Organizer: 第23回日本生殖免疫学会
  • Place of Presentation: 富山国際会言義場
  • Year and Date: 2008-12-07
• [Presentation] 着床期子宮局所におけるSTAT-3活性の役割 (2008)
  • Author(s): 中村仁美, 香山晋輔, 谷口武, 荻田和秀, 熊澤恵一, 張慶, 筒井建紀, 古山将康, 杉野法広, 田村博史, 金田安史, 木村正
  • Organizer: 第13回日本内分泌学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-11-29
• [Presentation] 着床期子宮内膜におけるマクロファージの役割 (2008)
  • Author(s): 中村仁美, Jasper MJ, Aplin JD, 香山晋輔, 荻田和秀, 熊澤恵一, 張慶, 筒井建紀, 木村正, Robertson SA.
  • Organizer: 第53回日本生殖医学会総会・学術講演会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-10-24
• [Presentation] 子宮局所における一過性遺伝子導入方法を用いた着床不全マウスモデルの作製 (2008)
  • Author(s): 香山晋輔, 中村仁美, 谷口武, 荻田和秀, 熊澤恵一, 張慶, 筒井建紀, 金田安史, 木村正
  • Organizer: 第53回日本生殖医学会総会・学術講演会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-10-24