2009 Fiscal Year Final Research Report
2-benzazepine derivative promotes skin epithelial cell migration and wound healing
| Project/Area Number |
20890144
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
General pharmacology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
MATSUURA Kenji Yamaguchi University, 大学院・医学系研究科, 助教 (20452641)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 皮膚 / 上皮 / 創傷治癒 / 細胞遊走 |
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| Research Abstract |
In search of biological activity of 2-benzazepine derivatives, we found that one of 2-benzazepine derivatives (compound A) promoted cell migration of human keratinocyte-derived cell line (HaCat cells) and wound healing of the skin in mouse. Here a structure-function relationship of 2-benzazepine derivatives was evaluated using in vitro wound healing assay (scratch assay) with HaCaT cells. A side chain at N-2 position was important for this biological activity of 2-benzazepine derivatives. The steric size between methyl and ethyl seems optimal at this side chain position, and longer alkyl chain than propyl group at N-2 position spoiled the biological activity. In the scratch assay, compound A -induced migration was blocked by the neutralizing antibody against transforming growth factor-beta (TGF-β), while neutralizing antibody against EGF had no effect. Those results indicate that compound A promotes the epithelial migration increasing the release of TGF-β.
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