2009 Fiscal Year Final Research Report
Gene expression analysis in small hepatocytes and cancer stem cells for searching cancer related genes.
| Project/Area Number |
20890179
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
OOE Hidekazu Sapporo Medical University, 医学部, 助教 (90452979)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 再生医学 / 癌 / 癌幹細胞 / 肝再生 / 小型肝細胞 |
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| Research Abstract |
Expression of hepatic stem cell markers such as CD24, CD34, CD44, CD133, was examined in 7 cell lines derived from rat hepatocellular carcinomas. Although CD44 was commonly expressed in all the cell lines, it is also expressed in hepatic progenitor cells, small hepatocytes (SHs), which possess no ability to form tumors. To investigate the regulatory mechanism of the gene expression, the method of gene transduction to rat SHs by lentivirus infection was established. Knockdown of Follistatin in SHs by shRNA resulted in the growth inhibition.
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