2009 Fiscal Year Final Research Report
Isolation of HSC progenitors in in vitro ES cell differentiation culture system.
| Project/Area Number |
20890285
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TANAKA Yosuke The Institute of Physical and Chemical Research, 幹細胞研究グループ, 客員研究員 (10509087)
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| Project Period (FY) |
2008 – 2009
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| Keywords | Runx1 / Gata1 / VE-cadherin |
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| Research Abstract |
We established RVG25 ES cell lines which can monitor expression of Runx1 and Gata1 at the same time. In the ES cell hematopoietic differentiation culture, we found that Runx1+Gata1+VE-cadherin+ cells had high ability to produce definitive hematopoietic cells (B cells). Additionally, we showed that VE-cadherin+ cells in E7.5 murine yolk sac could differentiate into B cells. Furthermore we made Gata1-IRES-MerCreMer mouse lines. Using the mice, we can label Gata1-expressing cells at any time.
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