2009 Fiscal Year Final Research Report
Genetic analysis of a guanine nucleotide exchanging factor involved in neural tube morphogenesis
| Project/Area Number |
20890292
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
TANEGASHIMA Kosuke Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 主任研究員 (20507678)
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| Project Period (FY) |
2008 – 2009
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| Keywords | 先天異常学 / 神経管閉鎖障害 / 発生・分化 / 脳・神経 / ノックアウトマウス |
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| Research Abstract |
Abnormal morphogenesis of the neural tube leads to neural tube defects including spina bifida and anencephaly. We found that a novel guanine nucleotide exchanging factor, WGEF mediates Wnt-PCP pathway, and regulates morphogenic movements of neural tube in the amphibian embryo. In this study, I attempted to generate a conditional knockout mouse of WGEF gene as a mammalian model of neural tube defects. So far, a conditional knockout allele on the WGEF locus was introduced by homologous recombination in the ES cells. Using this ES cells, chimera mice carrying WGEF conditional knockout cells have been generated.
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