Ecology and chemistry of plant-microbiome interactions in iron-deficient soil

2022 Fiscal Year Annual Research Report

• Project/Area Number: 20F20084
• Research Institution: Kyoto University
• Principal Investigator: 東樹 宏和 京都大学, 生態学研究センター, 准教授 (60585024)
• Co-Investigator(Kenkyū-buntansha): CANARINI ALBERTO 京都大学, 生態学研究センター, 外国人特別研究員
• Project Period (FY): 2020-09-25 – 2023-03-31
• Keywords: symbiosis / plants / mycorrhizal fungi

Outline of Annual Research Achievements

To finalize the research project, we have done the following experiments and analyses: 1) Experimental inoculation of endophytic fungi on model plant species: 2) Finalization of the tests for calibrating the necessary condition to successfully achieve stable isotope tracing techniques: these tests allowed us to characterize the necessary conditions to achieve measurable amounts of stable isotope tracer incorporated in different biomarkers for fungi and in plant biomass: 3) Carrying an in vitro experiment to test competition dynamics between different root endophytic fungi and arbuscular mycorrhizal fungi during nitrogen and carbon exchange with a model plant: this experiment allowed us to understand how endophytic fungi that carried similar function to AMF could modify the trading feature of carbon and nitrogen exchange in the AMF-plant symbiosis: 4) Carrying a pot experiment to validate the in vitro results: this experiment allowed us to generalize the findings obtained in in vitro conditions to soil-grown plants: 5) Transcriptomic analyses (RNA sequencing) of the model plant specimens used for the inoculation experiments of endophytic fungi. 6) In-silico analyses of the RNA-sequencing datasets mentioned above.

Research Progress Status

令和4年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和4年度が最終年度であるため、記入しない。