2021 Fiscal Year Annual Research Report
Fabrication of 3D in vitro model of diabetic neuropathy with real-time detection of ROS generation
| Project/Area Number |
20F20361
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| Research Institution | The University of Tokyo |
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Principal Investigator |
竹内 昌治 東京大学, 大学院情報理工学系研究科, 教授 (90343110)
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| Co-Investigator(Kenkyū-buntansha) |
MYASNIKOVA DINA 東京大学, 情報理工学(系)研究科, 外国人特別研究員
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| Project Period (FY) |
2020-11-13 – 2023-03-31
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| Keywords | skin / vascular structure / neurons / pancreatic islets |
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| Outline of Annual Research Achievements |
This year I made some minor improvements in the device for culturing skin equivalent, improved skin equivalent culture protocol, which led to better barrier function, characterized by TEER and permeation test. I have leant how to operate HPLC, so now I can do it by myself.
Also, I have learnt how to work with iPS cells and successfully differentiated them into sensory neurons, which was confirmed by immunofluorescence staining. Furthermore, I incorporated sensory neurons and vascular cells into pancreatic spheroids and confirmed formation of neuron network and immature vasculature inside spheroids.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
I see great progress in my research, for which I am grateful for my supervisor and lab members, they provide enormous support for me. I did not achieve all of the goals I wanted, but the reason for that was that I overestimated how much can be done in 1 year. There were a lot of small hiccups and research questions I did not envisioned when I was writing my plan. And this is also a great chance for me to learn as a scientist, so that I can be more realistic next time.
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| Strategy for Future Research Activity |
I will continue my work according to my plan. This year I will incorporate neuron network into the structure of perfusable skin equivalent with vascular like tubes. I also want to improve the protocol I am using for fabrication of neurovascularised pancreatic spheroids, as I was able to fabricate neuron network inside spheroids, but vasculature was immature. And this year I will connect two tissues in one Organ on a chip system. I also want to test some medicines that are used for Diabetic Neuropathy and see their influence on my skin model.
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