2021 Fiscal Year Annual Research Report
Integrated OMICS Study of the Beneficial Effects of Egg Shell Membrane-derived Resistant Protein on Intestinal Bacteria
| Project/Area Number |
20F20401
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| Research Institution | The University of Tokyo |
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Principal Investigator |
加藤 久典 東京大学, 大学院農学生命科学研究科(農学部), 特任教授 (40211164)
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| Co-Investigator(Kenkyū-buntansha) |
YANG YONGSHOU 東京大学, 農学生命科学研究科, 外国人特別研究員
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| Project Period (FY) |
2020-11-13 – 2022-03-31
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| Keywords | registant protein / eggshel membrane / microbiota / sake / IgA / high fat diet |
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| Outline of Annual Research Achievements |
Consumption of resistant proteins (RP: non-digestible proteins) has been reported to exert beneficial functions. However, the involvement of gut microbiota in such beneficial effects is not fully elucidated. Previous studies have shown eggshel membrane (ESM) and sake cake (Profiber: PFB) contain a large amount of RP. Here, I performed the first animal experiment to investigate the effects of dietary ESM or PFB on metabolic dysfunctions and gut dysbiosis induced by high-fat diet (HFD).
A total of 48 C57BL6J male mice was assigned randomly to the following six dietary groups: (1) Control diet (Ctrl), (2) high-fat diet (HFD), (3) HFD with 8% ESM (HESM), (4) HFD with 8% ESM+ antibiotics mix (ESMAbx), (5) HFD with 30% PFB (HPFB), (6) HFD with 30% PFB + antibiotics mix (PFBAbx). Compared with Ctrl group, mice fed an HFD showed increased body weight, visceral fat mass, liver weight, liver triglycerides, and liver cholesterol. Compared with the HFD group, the mice body weight gains were reduced in both of HESM and ESMAbx groups. However, the mice body weight gains were not affected in HPFB and PFBAbx groups compared that in the HFD group. The oral glucose tolerance test was not affected in these treatment groups. PFB treatment reduced the level of cholesterol in serum and liver. Compared with the Ctrl group, HFD showed increased level of immunoglobulin (IgA) in colon content. ESM treatment did not affect IgA level in colon content compared with that of HFD group. PFB treatment was shown to slightly increase the IgA level in colon content.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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[Journal Article] Dietary eggshell membrane powder improves survival rate and ameliorates gut dysbiosis in interleukin-10 knockout mice2022
Author(s)
Yang, Y., Jia, H., Furukawa, K., Li, X., Hasebe, Y. and Kato, H.
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Journal Title
Front. Nutr.
Volume: 9
Pages: 895665
DOI
Peer Reviewed / Open Access
