2023 Fiscal Year Final Research Report
Elucidation of nano-mechanical properties of a highly adhesive and highly extensible protein and creation of mechanochemical materials
| Project/Area Number |
20H00319
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 27:Chemical engineering and related fields
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤本 和士 名古屋大学, 工学研究科, 助教 (70639301)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ナノファイバータンパク質 / 強接着 / 高伸展性 / ナノ力学 / メカノケミカル材料 |
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| Outline of Final Research Achievements |
In this study, we performed a detailed analysis of the kinetic behavior of the bacterionanofiber protein AtaA, which exhibits strong adhesion and high extensibility, using atomic force microscopy and molecular dynamics simulations. Each domain of AtaA showed a completely different mechanical response, with some domains being tougher than the muscle protein titin. By combining and analyzing the measurement results with the simulation data, we clarified the nanomechanical response and mechanisms of proteins with complex quaternary structures.
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| Free Research Field |
生物工学
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| Academic Significance and Societal Importance of the Research Achievements |
蛋白質の力学挙動、換言するとナノレオロジーは世界で注目の研究分野であるが、AtaAのような多種類のβ構造がコイルドコイルで連結された複雑な四次構造を持つ多量体蛋白質の解析事例はなく、重要かつ新たな知見を与えるものである。また本研究の知見を基に、特定の力で伸展シグナルを検知する仕組みを導入したり、望ましい力学特性を示すドメインを連結して超高分子量繊維や蛋白質ゲルを作出することで、新奇メカノセンサーやメカノバイオロジー操作基材開発への応用が期待できる。
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