2023 Fiscal Year Final Research Report
Elucidation of metabolic function of microbial dark matter CPR bacterial population in activated sludge and its isolation
| Project/Area Number |
20H02287
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 22060:Environmental systems for civil engineering-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
青井 議輝 広島大学, 統合生命科学研究科(先), 准教授 (40386636)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 微生物ダークマター / CPR細菌群 / Patescibacteria / 分離培養 |
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| Outline of Final Research Achievements |
We selected activated sludge with relatively high Patescibacteria abundance, extracted DNA from activated sludge samples, and reconstructed 10 high-quality Patescibacteria genomes by metagenomic analysis. Furthermore, the full length of the 16S rRNA gene sequence in the Patescibacteria genome was extracted, and fluorescent in situ hybridization (FISH) probes specific for Patescibacteria were successfully designed using the phylogenetic tree generation software ARB. by FISH method. The results suggest that bacterial cells in the phylum BD1-5 exist near the cells hybridized with Zoogloea spp. present in activated sludge, suggesting that they have established a parasitic-symbiotic relationship.
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| Free Research Field |
排水処理工学
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| Academic Significance and Societal Importance of the Research Achievements |
既往のメタゲノム解析では、あるPatescibacteriaはゲノムサイズが非常に小さく、生命に必須の呼吸や核酸合成などの遺伝子群が欠落していることが分かっているPatescibacteriaが活性汚泥を含めてどのように生存しているかを解明することは、38億年前の地球上の初期生命の進化の理解へとつながり、微生物ダークマターの謎にせまることができる。メタゲノム解析は環境微生物学の分野で主流になっているが、ゲノム情報からの推察では不十分な点が多い。特に本研究のように可視化することで細胞サイズや他細菌との共生関係など分離培養へと繋がる情報が得られたことの意義は大きい。
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