Elucidation of the mechanism in disease protection of the fish epidermis-derived probiotics.

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02539
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Nagoya University
• Principal Investigator: Nakatani Hajime 名古屋大学, 工学研究科, 講師 (80456615)
• Co-Investigator(Kenkyū-buntansha): 堀 克敏 名古屋大学, 工学研究科, 教授 (50302956)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: プロバイオティクス / 細菌叢 / 魚病

Outline of Final Research Achievements

It has been shown that multiple administrations of the bacterium Pseudomonas mosselii KHZF1, which suppresses the growth of fish pathogens, into water, have an effect on suppressing pathogenic infections in fish. The effect is suggested to be due to a significant increase in the proportion of KHZF1 in the fish's skin microbiota by administration into the water and the antibacterial substances produced by KHZF1. Attempts to identify the antibacterial substances produced by KHZF1 have revealed that the antibacterial substance is a known substance called Pseudoiodinine, which showed inhibitory effects on the growth of various fish pathogens at low concentrations. Further research is needed to clarify the role of the antibacterial substance in the manifestation of disease control effects.

Free Research Field

応用微生物学

Academic Significance and Societal Importance of the Research Achievements

広い科学の観点から魚介類表皮プロバイオティクス技術の基盤となる研究は、魚介類の表面をモデルとしたホスト-微生物叢間の相互作用に関する基礎的研究ともいえる。次世代シーケンス技術の発達によりDNAの網羅的なシーケンスが可能となって以来、動物や植物の表面や、環境に形成されている微生物叢がそれらの動植物の健康や生態系サービスにとって重要な役割を持つことが明らかになっている。このように現在、微生物叢の構成の重要さが様々な分野で認識されつつあるなかで、本研究開発で得られた知見は、将来的に魚介類の養殖にとどまらず、環境・医療・農業・水産など様々な分野での活用がみこまれる。