2022 Fiscal Year Annual Research Report
Critically testing magnetoreception mechanisms at the cellular level
| Project/Area Number |
20H02687
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ウッドワード ジョナサン 東京大学, 大学院総合文化研究科, 教授 (80526054)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 磁気受容 / 細胞の自家蛍光 / ラジカル反応 / 磁場効果 / 発光分析 / スピン化学 |
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| Outline of Annual Research Achievements |
The proof of principle of an advanced new radical pair based microscopy technique, Pump Field Probe (PFP) microscopy that allows the direct detection of transient spin correlated radical pairs by fluorescence microscopy was robustly tested experimentally. Our previous work on magnetic field effects on autofluorescence in living cells demands new techniques to provide detailed spectroscopic and structural information on the radical pairs involved and no existing techniques can provide this information. This work is now being prepared for publication.
An article was published on Bioarxiv which aimed to reproduce our magnetic field effects on cellular autofluorescence (due to its significance) but which was unable to do so. A detailed investigation of the techniques used was undertaken along with theoretical simulations which demonstrated that the irradiation conditions employed in the reproduction experiment were not suitable to observe the magnetic field dependence. This response was published on BioarXiv.
Full model system testing of the multi-wavelength TOAD microscope was completed with improved signal-to-noise ratio achieved over the original single wavelength implementation. New Labview based software to control the instrument was completed and which allows automatic switching between probe laser wavelengths, automatic detector balancing and automatic bandpass filter switching.
Theoretical simulations were conducted for zero field triplet eigenstates in multinuclear systems.
An invited article on magnetic field effects in biology was written and published.
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| Research Progress Status |
令和4年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和4年度が最終年度であるため、記入しない。
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