Development of Frequency Division Multiplex HPLC-MS/MS for High Performance Label Free Differential Analysis

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02763
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 34020:Analytical chemistry-related
• Research Institution: Nagoya Institute of Technology
• Principal Investigator: Kitagawa Shinya 名古屋工業大学, 工学(系)研究科(研究院), 教授 (50335080)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 質量分析 / 液体クロマトグラフィー / ラベルフリー / ディファレンシャル解析 / 周波数分割多重化 / 並列分析

Outline of Final Research Achievements

In this study, we successfully developed a multiplex mass spectrometry system capable of six samples, and demonstrated that quantitative differential analysis can be made with high accuracy by using suitable internal standards. We also demonstrated that the frequency division multiplex MS method can be expanded to MS/MS measurements and to an alternating measurement mode in which MS measurements with and without collision induced decay are performed alternately. That is, we succeeded in demonstrating that quantitative comparison in 6LC-1MS/MS is possible. In addition, we also investigated on a sample injection system and a pump system for 6-parallel LC analysis, a micro-scale LC system to improve the stabilities of ionization efficiency and ion introduction efficiency, and a procedure for high performance signal processing in the alternating measurement mode.

Free Research Field

分析化学

Academic Significance and Societal Importance of the Research Achievements

オミクス解析では、疾患の有無などのサンプル群間における化合物量（タンパク質発現量など）の差異を明らかにするディファレンシャル解析（定量比較解析）が必須である。近年、液体クロマトグラフと質量分析器(MS/MS)を用いるディファレンシャル解析の重要性は増大しておりその高効率化が求められているが、本研究はこれに貢献するものである。また、本研究の根幹は並列導入型MS/MSであるため、MS(/MS)を用いるすべての分析（臨床分析、環境分析、生体分析など）の高効率化に貢献できる。MS（/MS）が必要とされる大規模調査の高効率化・低コスト化にも貢献することができる。