2022 Fiscal Year Final Research Report
Photon Upconversion Fluorescent Probe for Sequence Selective Detection/Imagine of Double-Stranded DNA
| Project/Area Number |
20H02768
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Kyushu University |
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Principal Investigator |
Nakano Koji 九州大学, 工学研究院, 准教授 (10180324)
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| Co-Investigator(Kenkyū-buntansha) |
石松 亮一 九州大学, 工学研究院, 助教 (90512781)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 蛍光プローブ / フォトンアップコンバージョン / 二重鎖DNA / 分子イメージング / エピジェネティック |
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| Outline of Final Research Achievements |
We report a new fluorescent probe that utilizes triplet-triplet annihilation-based photon upconversion. In particular, for analyses of the androgen-responsive sequence (ARE) in genomic DNA, we designed a type of ARE-binding pyrrole-imidazole polyamides incorporating Pt-coproporphyrin (PtCP) and anthracene (ANTH). We successfully developed a practical synthetic route in the first half of the research period. Subsequently, we studied the binding behavior to the ARE fragment and measured the fluorescence spectra for the ARE-DNA probe using a laser excitation fluorescence spectrophotometer. At last, we found that when the PtCP in the molecule was excited with excitation light at 533 nm, the molecule gave the phosphorescence of PtCP (650 nm) and the fluorescence from ANTH (480 nm). Therefore we concluded that, for the first time, we successfully developed a photon upconversion probe for DNA analyses.
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| Free Research Field |
分析化学
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| Academic Significance and Societal Importance of the Research Achievements |
基本的な励起一重項からの発光をはじめ蛍光共鳴エネルギー移動、遅延発光を利用する方法など、蛍光プローブは細胞のin vivo観察で成果を収めてきた。しかし、可視領域の蛍光イメージを得るためには強力な紫外光励起が必要で、細胞は深刻なダメージを受けてしまう。このような状況のもと、低損傷な長波長の可視光で励起でき、より短波長側で蛍光を示すDNA結合性蛍光プローブを開発した。これは世界で初めての例であり学術的な意義は大きい。また、二光子励起蛍光法のように取扱いが難しく、大掛かりで高価な装置は必要ないので、実用化の後には、ベンチトップ観察法として細胞研究や医療診断に貢献できる点で社会的な意義は大きい。
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