Advanced phage-display method with high efficiency by magnetic orientation of rod-like viruses

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02791
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 35010:Polymer chemistry-related
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ishida Yasuhiro 国立研究開発法人理化学研究所, 創発物性科学研究センター, チームリーダー (20343113)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ファージディスプレイ法 / 分子進化工学 / 親和性選択 / ライブラリ / 磁場配向

Outline of Final Research Achievements

Although phage display selection using a library of M13 bacteriophage has become a powerful tool for finding peptides that bind to target materials on demand, a remaining concern of this method is the interference by the M13 main body, which is a huge filament >1000 times larger than the displayed peptide, and therefore would nonspecifically adhere to the target or sterically inhibit the binding of the displayed peptide. Meanwhile, filamentous phages are known to be orientable by an external magnetic field. This work proved that the magnetic orientation of M13 filaments vertical to the target surface significantly affects the selection. When the target surface was affinitive to the M13 main body, this orientation notably suppressed the nonspecific adhesion. Furthermore, when the target surface was less affinitive to the M13 main body and intrinsically free from the nonspecific adhesion, this orientation drastically changed the population of M13 clones selected from the library.

Free Research Field

材料化学　超分子化学　高分子化学

Academic Significance and Societal Importance of the Research Achievements

2018年ノーベル化学賞の対象ともなったファージディスプレイ法が、その創設以来抱える問題（巨大なファージ外殻による吸着選択効率の低下）に対し、化学物質の添加を必要とせずにクリーンな物理的刺激のみを用い、様々な標的物質に対し適応できる解決法を提示した。