Detection of biomolecules by using chiral amplification system composed of artificial nucleic acids

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02858
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Nagoya University
• Principal Investigator: Kashida Hiromu 名古屋大学, 工学研究科, 准教授 (30452189)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: キラル増幅 / 人工核酸 / 円偏光発光 / 円二色性

Outline of Final Research Achievements

In this study, we aimed to develop a chiral amplification system utilizing serinol nucleic acid (SNA). First, we prepared a one-dimensional nano structure by hybridizing achiral SNA strands. When chiral nucleic acid was added, CD intensity drastically increased. Thus, chiral amplification system triggered by duplex formation was successfully developed for the first time. Then we modified SNA nano structures with pyrene. The structure expressed circularly polarized luminescence (CPL) upon the addition of chiral nucleic acids. We also succeeded to develop a chiral amplification system triggered by DNA.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

生体分子はほぼ全てキラルであるため、キラリティに基づいた検出法を開発することが出来れば、生体分子を検出する非常に汎用性の高い手法となることが期待できる。本研究では、核酸のキラリティを増幅し、円二色性や円偏光発光によって検出する全く新しい手法を開発した。今後、これを利用した天然核酸検出や他の生体分子検出への応用が期待できる。