Understanding Cellular Function with Short RNAs and Small Molecules

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02859
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Kyoto University
• Principal Investigator: SATO SHINICHI 京都大学, 化学研究所, 准教授 (70534478)
• Co-Investigator(Kenkyū-buntansha): 萩原 正規 弘前大学, 理工学研究科, 准教授 (40403000)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 機能性RNA / 小分子化合物 / ケミカルバイオロジー / イメージング

Outline of Final Research Achievements

To understand complex cellular functions, it is important to systematically investigate the spatio-temporal functional expression of bioorganic molecules such as proteins and nucleic acids. However, there are few methods that allow analysis of endogenous bioorganic molecules in living cells, making it technically difficult. In this research, we established an innovative method to regulate RNA functions in mammalian cells and to analyze gene expression by using "a functional RNA and a small molecule" as a research tool.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

本研究では生細胞内でRNA機能を操る新たな研究手法を創出し、未知の生命現象を解き明かす研究ツールとして利用することを目指した。研究期間内で大きな成果を得ることに成功し、国際的評価が最も高い核酸化学専門誌であるNucleic Acids Research誌（IF＝19.160）への２報の報告を含む、４報への国際誌に研究成果を報告した。
Selected Publications
1. Nucleic Acids Research,50(14),8143-8153,2022, 2. Nucleic Acids Research,49(22),e132-e132,2021