2022 Fiscal Year Final Research Report
Development of gene-directed caged compounds
| Project/Area Number |
20H02882
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 37030:Chemical biology-related
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| Research Institution | Toho University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ケージド化合物 / 光スイッチ / 光薬理学 / クリックケミストリー / エピジェネティクス / 抗菌薬 |
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| Outline of Final Research Achievements |
Our goal was to create innovative experimental techniques to better understand higher brain functions like memory and learning, as well as the molecular mechanisms behind complex diseases such as cancer. We successfully designed gene-directed caged compounds that overcome limitations of existing ones and combine the benefits of optogenetics and small molecular probes. Through our research, we demonstrated the ability to regulate intracellular signaling and epigenetics in mammalian cells using exogenous enzymes like galactosidase from Escherichia coli and porcine liver esterase. Additionally, we developed clickable caged compounds that allow for easy integration of functional components through click reactions. These compounds were utilized to create photoactivated prodrugs of anticancer drugs, conjugated with cell type-selective ligands.
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| Free Research Field |
ケミカルバイオロジー
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| Academic Significance and Societal Importance of the Research Achievements |
記憶や学習などの脳の高次機能の解明，および，がんに代表される難治性疾患発症の分子機構の解明に貢献する技術開発を目指して，低分子量有機化合物（薬剤）とタンパク質の利点を併せ持つ光作動性機能性分子の設計・合成に成功した。光スイッチと遺伝子組み換え技術を利用して，任意の標的細胞内だけで，各種薬剤を選択的に機能させる光薬理学への道を拓くことができた。ドラッグデリバリーの新手法としても期待できる。
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