Conversion of legume crops to low carbon input lines using nitrogen fixation enhancing genes

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H02890
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 38010:Plant nutrition and soil science-related
• Research Institution: Saga University
• Principal Investigator: Suzuki Akihiro 佐賀大学, 農学部, 教授 (50305108)
• Co-Investigator(Kenkyū-buntansha): 渡邊 啓史 佐賀大学, 農学部, 准教授 (40425541) ; 富永 晃好 静岡大学, 農学部, 助教 (50776490) ; 下村 彩 佐賀大学, 農学部, 助教 (20802771) ; 遠城 道雄 鹿児島大学, 農水産獣医学域農学系, 教授 (60194651) ; 穴井 豊昭 佐賀大学, 農学部, 招へい教授 (70261774)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 根粒菌 / 窒素固定 / ダイズ / 収量 / 遺伝子 / 共生

Outline of Final Research Achievements

In this research, we used a FUKUYUTAKA SEN1 line (Enrei type) and a conventional line with the SEN1 gene (Peking type), and conducted three research projects: (1) verification of the effect of the Enrei type on nitrogen fertilizer reduction, (2) the effect of the Enrei type on seed　yield, and (3) functional analysis of the SEN1 protein. As a result, it was found that (1) the Enrei type showed the same growth as the Peking type even when nitrogen fertilizer was reduced to half. In case (2), the nitrogen fixation enhanced SEN1 showed a tendency to increase yield more than the Peking type in nitrogen poor condition. In (3), the higher concentration of iron in the nodules of the Enrei type supported the possibility that the SEN1 protein is involved in iron transport.

Free Research Field

作物生理学

Academic Significance and Societal Importance of the Research Achievements

ダイズにおける窒素固定増強遺伝子を同定し，それを日本で最も栽培面積の大きな品種（フクユタカ）に導入し，その性状解析を行った点は，学術的に大きな意義がある。また得られた結果は，ダイズ栽培においてこの遺伝子を用いれば，窒素肥料の半分程度までの低減を達成できる可能性を示すものであり，肥料価格が高騰している現代社会において非常に大きなインパクトのある成果だと考えられる。