2022 Fiscal Year Final Research Report
Elucidation of the gene expression profile of bifidobacteria in the gut microbiota: development of selective recovery method
Project/Area Number |
20H02893
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
|
Research Institution | Hokkaido University |
Principal Investigator |
Fukiya Satoru 北海道大学, 農学研究院, 教授 (10370157)
|
Co-Investigator(Kenkyū-buntansha) |
後藤 恭宏 九州大学, 医学研究院, 助教 (20558358)
|
Project Period (FY) |
2020-04-01 – 2023-03-31
|
Keywords | ビフィズス菌 / 選択的回収 / 腸内細菌叢 / 腸内生存 / トランスクリプトーム |
Outline of Final Research Achievements |
Molecular mechanisms of how bifidobacteria survive in the intestine are not fully understood especially in the presence of the gut microbiota. To clarify the mechanisms, we tried to establish an experimental system to collect Bifidobacterium longum administered into the conventional mouse intestine selectively to obtain its gene expression profiles in the presence of gut microbiota. Four kinds of B. longum strains expressing different peptide tags on the cell surface were constructed as a fusion with the surface-presenting protein. After verifying the tag expression and surface presentation, we established an optimum collection method in vitro using the Strep-tagII. In addition, the tag-expressing strain was successfully recovered from the artificial gut microbiota using the established collection method.
|
Free Research Field |
応用微生物学
|
Academic Significance and Societal Importance of the Research Achievements |
本研究では,ペプチドタグを細胞表層に発現するビフィズス菌株を構築し,人工腸内細菌叢からビフィズス菌を選択的に回収する方法を確立した.これまで腸内細菌叢存在下でのビフィズス菌の詳細な遺伝子発現プロファイルは得られていないが,本研究の成果はその情報を得るためのブレークスルーとなる.また本研究の今後の進展により,ビフィズス菌の腸内での生存機構を明らかにすることが期待される.これにより,様々な健康効果をもたらすビフィズス菌を腸内でどのように維持し,人々の健康維持に貢献するかという社会的な課題に応える基盤的な情報を得ることができる.したがって,本研究の成果は社会的にも重要な成果であると評価できる.
|