2023 Fiscal Year Final Research Report
Comprehensive understanding of glucose inactivation in the conversion from respiratory metabolism to fermentation in yeast
| Project/Area Number |
20H02894
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤田 翔貴 東北大学, 農学研究科, 助教 (70845099)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 出芽酵母 / グルコース不活性化 / エンドサイトーシス / ユビキチン化 / 輸送体 / 液胞 / 転写因子 |
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| Outline of Final Research Achievements |
The yeast Saccharomyces cerevisiae used in fermentation undergoes a major metabolic shift between “fermentation” and “aerobic respiration” depending on the type of carbon source. During the transition from aerobic respiration to fermentation, glucose inactivates the glycolytic pathway. In addition to metabolic enzymes, lactate transporters, acetate transporters, and succinate-fumarate transporters are newly found to be inactivated. Furthermore, amino acid residues required for endocytosis of Gal2 (galactose transporter) and Hxt6 (high-affinity glucose transporter), which are inactivated in response to glucose stimulation, were identified. The role of SNARE protein Ykt6 in the biogenesis of vacuoles responsible for degradation of membrane transporters was clarified.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
細胞は栄養源などの外界シグナルに応答し、細胞のリモデリングを行って新たな環境に適応している。リモデリングは細胞成分の合成と分解の平衡によって成り立つ。本研究では、酵母の人類社会における最も重要な役割であるアルコール発酵に着目し、非発酵期から発酵期への転換におけるリモデリングをタンパク質分解に焦点を当てて解析した。糖新生経路における化合物の変換に加え、物質の移動を制御することにより適応する姿が明らかとなった。
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