2022 Fiscal Year Final Research Report
Elucidation of survival strategy of yeast by comprehensive analysis of genes preferentially expressed under translational repression stress.
Project/Area Number |
20H02900
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | Kyoto Institute of Technology |
Principal Investigator |
Izawa Shingo 京都工芸繊維大学, 応用生物学系, 准教授 (10273517)
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Co-Investigator(Kenkyū-buntansha) |
赤尾 健 独立行政法人酒類総合研究所, 研究部門, 部門長 (50416426)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | 翻訳抑制ストレス / エタノールストレス / 出芽酵母 / DUMPs / Hsp78 / Deposition sites / Protein quality control / 醸造過程 |
Outline of Final Research Achievements |
Severe stress, such as high ethanol concentration and glucose depletion, suppresses the translational activity of yeast cells, and most mRNAs are sequestered in stress granules and processing bodies without being translated. On the other hand, mRNAs that are crucial for coping with the emergency situation of severe stress are exceptionally and preferentially translated and play an important role in adaptation to severe stress and survival. We have succeeded in identifying a number of genes that are preferentially translated and expressed under high ethanol stress and have proceeded to analyze their functions. Some of them were Protein Quality Control (PQC)-related factors that cope with protein denaturation induced by severe ethanol stress.
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Free Research Field |
微生物生理学
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Academic Significance and Societal Importance of the Research Achievements |
高濃度エタノールストレス下および醸造過程の変性タンパク質レベルについて検討を行い、不溶性タンパク質レベルが上昇し蓄積すること、変性タンパク質の隔離場所deposition sitesが形成されること、ミトコンドリアでは変性タンパク質の隔離・再生の場であるDUMPsが形成されることを明らかにした。また、DUMPsで機能するHsp78が高濃度エタノール下で優先的に発現することを確認した。さらに、醸造過程では、変性タンパク質の隔離・再生のステップが高濃度エタノールへの対処に重要であることを明らかにした。また、発酵温度によるPQC関連因子の発現レベルや活性の違いについても、新たな現象を見出した。
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