2022 Fiscal Year Final Research Report
Periplasmic metabolic engineering in acetic acid bacteria
| Project/Area Number |
20H02902
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
阿野 嘉孝 愛媛大学, 農学研究科, 准教授 (00403642)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 応用微生物学 / 発酵 / バイオテクノロジー / 酵素 / 酢酸菌 / 代謝工学 |
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| Outline of Final Research Achievements |
Acetic acid bacteria have a unique oxidation system on the cell surface and therefor, have been used for acetic acid fermentation and also sorbose fermentation in vitamin C production process. Considering that metabolism in the periplasmic space, which is the intracellular compartment of bacteria, has advantages over metabolism in the cytoplasm, this aimed at investigation for efficacy of "periplasmic metabolic engineering" that expands metabolism in the periplasm. The first attempt was to relocate a dehydratase (lyase), which is localized in the cytoplasm in the acetic acid bacteria, to the periplasm. Next, we tried to translocate isomerase, which is naturally localized in the cytoplasm. Both periplasmic metabolic engineering increased the biotransformation rate. Expression of the protein involved in transport to the periplasm also increased the bioconversion rate.
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| Free Research Field |
応用微生物学
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| Academic Significance and Societal Importance of the Research Achievements |
持続可能な発展的経済活動の必要性や二酸化炭素排出低減が訴えられている中，微生物を用いた物質生産は廃棄物利用や環境負荷の低減という意味で魅力的なソリューションの一つである。本研究は，古くから物質生産に利用されている酢酸菌を用いた，新しい概念の代謝工学の検証を行った。いくつかの良好な結果を得ることができたので，当初期待していた新しい代謝工学の有効性を確認することができた。本菌でのさらなる展開はさることながら，大腸菌などの他の微生物を用いた展開が想定できる。この研究が，広く微生物による代謝工学の考え方を変え，新しい物質生産系の構築という形で波及していくことを期待する。
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