2023 Fiscal Year Final Research Report
Molecular mechanisms of flexible plant immunity by receptors for reactive oxygen species
| Project/Area Number |
20H02984
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 39040:Plant protection science-related
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Keywords | 活性酸素種 / ROSセンサー / 植物免疫 |
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| Outline of Final Research Achievements |
ROS bursts are induced by RBOH in the plasma membrane. ROS has been observed as a marker of defense response, but its mechanism of action is not known. ROS sensor protein was purified as a YAP1-binding protein. A wide variety of 102 ROS sensor candidates were obtained. Among these candidates, the recombinant protein of NbGLR was treated with H2O2, then the protein was chemically labeled with a BTD that binds to the sulfenylated cysteine residue. The sulfenylation of the target cysteine residue was confirmed in vitro using a specific antibody.
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| Free Research Field |
植物免疫学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、ROSの作用機構を標的タンパク質の酸化による構造変化に基づいて明らかにする試みであり、植物免疫の実態を突き止めることができるものと考えられる。作物生産においては、殺菌性農薬に依存して生産量を確保しているが、環境負荷に懸念が持たれている。このような状況において、作物に免疫を附与する免疫誘導剤の開発が急務である。本研究で得られる成果は、ROSによる抵抗性や免疫細胞死の制御機構を提供するのみでなく、新たな植物免疫誘導剤の開発に貢献するものである。
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