Identification and mode of action of novel plant defense activators that promote accumulation of jasmonic acid in plants

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H02990
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Tokyo University of Science
• Principal Investigator: Kuchitsu Kazuyuki 東京理科大学, 創域理工学部生命生物科学科, 教授 (50211884)
• Co-Investigator(Kenkyū-buntansha): 倉持 幸司 東京理科大学, 理工学部応用生物科学科, 教授 (90408708)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 抵抗性誘導剤 / 植物免疫 / ジャスモン酸 / サリチル酸 / 構造活性相関 / 病虫害耐性

Outline of Final Research Achievements

Using high throughput screening based on the ROS production in tobacco BY-2 cells, we identified a novel putative plant defense activator that enhances accumulation of both jasmonic acid (JA) and salicylic acid (SA) in Arabidopsis. Although JA- and SA-signaling pathways are mutually antagonistic in general, transcriptomic analyses revealed that it induces the expression of genes related to the biosynthesis and downstream of JA and SA. We systematically synthesized a series of related compounds to analyze the structure-activity relationship and labeled compounds to identify cellular target sites. We showed potential utility of this compound as a lead for plant defense activators as well as a chemical biological tool to dissect plant signaling network.

Free Research Field

植物分子生理学・植物免疫学

Academic Significance and Societal Importance of the Research Achievements

植物ホルモンであるジャスモン酸(JA)・サリチル酸(SA)は、一般に拮抗的に作用すると考えられているが、双方の蓄積を誘導し双方の経路を活性化する化合物を発見した。植物の病虫害抵抗性誘導剤、特化代謝促進剤などのリード化合物や、植物免疫系研究のツールなど、多様な応用の可能性が期待される。