2022 Fiscal Year Final Research Report
Impacts of lignin structural variations on the functions and properties of lignocellulose as studied with lignin-modified engineered plants
| Project/Area Number |
20H03044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 40020:Wood science-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
堀 千明 北海道大学, 工学研究院, 助教 (50722948)
久住 亮介 京都大学, 農学研究科, 助教 (70546530)
梅澤 俊明 京都大学, 生存圏研究所, 教授 (80151926)
今井 友也 京都大学, 生存圏研究所, 教授 (90509142)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Lignin / lignocellulose / cell wall / bioengineering / supramolecular structure / NMR / X-ray / genome editting |
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| Outline of Final Research Achievements |
The structural diversity of lignin in secondary cell walls is closely related to the evolution and environmental adaptation of vascular plants, whereas it also acts as a key inhibitory factor for the utilization of lignocellulosic biomass. The objective of this study was to elucidate the biosynthetic mechanism of lignin monomers that contribute to the formation of diverse lignin substructures. Additionally, we aimed to establish the relationship between the molecular structure of lignin and the functions and properties of lignocellulose. To achieve these goals, we identified several enzyme genes involved in the biosynthesis of the diverse lignin monomers in rice and engineered the identified genes to generate new lignin-modified rice mutant and transgenic lines. Subsequently, we examined the supramolecular structure and biomass properties of lignocellulose from the developed rice mutants and transgenic lines to investigate the impacts of the altered amount and structure of lignin.
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| Free Research Field |
木質科学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果は、維管束植物の進化や環境適応の仕組みの理解に繋がる細胞壁の機能発現におけるリグニンの役割の理解に重要な基盤を与えるものである。同時に、循環型社会構築に向けた木質バイオマス利用におけるリグニンによる阻害機構の理解や代謝工学や分子育種を通じた利用性を向上させたバイオマス生産植物の開発に寄与する知見が得られた。
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