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2023 Fiscal Year Final Research Report

Cryo-EM Study of Rotation Mechanism of V-type ATPase

Research Project

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Project/Area Number 20H03231
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43040:Biophysics-related
Research InstitutionKyoto Sangyo University

Principal Investigator

Yokoyama Ken  京都産業大学, 生命科学部, 教授 (70271377)

Co-Investigator(Kenkyū-buntansha) 光岡 薫  大阪大学, 超高圧電子顕微鏡センター, 教授 (60301230)
Project Period (FY) 2020-04-01 – 2023-03-31
KeywordsATP / V-ATPase / ATP synthase / molecular motor / CryoEM / bioenergetics / structure biology
Outline of Final Research Achievements

Life contains rotational molecular motor proteins that operate by spinning. These proteins are crucial membrane proteins that maintain cellular homeostasis through ATP (adenosine triphosphate) production and ion transport. Using cryo-electron microscopy (cryo-EM), we captured snapshots of the rotation of V/A-ATPase, a rotary molecular motor protein. By stitching these snapshots together, we revealed how V/A-ATPase rotates, fueled by ATP. This updated model provides valuable insights into the remarkable molecular machinery created by nature.Furthermore, we were able to elucidate the structural basis for the uni-site catalytic mechanism that occurs at one of the three catalytic sites.

Free Research Field

生物物理学

Academic Significance and Societal Importance of the Research Achievements

本研究により、ATPで駆動する分子モータータンパク質の仕組みに関する重要な知見を得ることができた。また、ユニサイト触媒の分子基盤、定常状態へと移行する過程を解明することができた。このナノサイズで機能する分子機械の仕組みを理解することで、将来のナノマシーンの駆動部に応用できる人工分子モータータンパク質設計への応用が期待される。
同じ方法で、他のタンパク質の仕組みを解明することが可能であり、今回の研究は、新しいタンパク質研究の世界を切り開く先駆けになる。

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Published: 2025-01-30  

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