Mechanism of growth inhibition by overexpression explored from the expression level of the limiting mutant protein

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H03242
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43060:System genome science-related
• Research Institution: Okayama University
• Principal Investigator: Moriya Hisao 岡山大学, 環境生命自然科学学域, 教授 (60500808)
• Co-Investigator(Kenkyū-buntansha): 牧野 能士 東北大学, 生命科学研究科, 教授 (20443442)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 酵母 / 過剰発現 / タンパク質 / 細胞毒性

Outline of Final Research Achievements

Overexpression of proteins can harm cells in various ways. Using budding yeast as a model for eukaryotic cells, we have investigated the characteristics of protein groups that cause growth inhibition when overexpressed and have classified the mechanisms of growth inhibition. In this study, we further explored the toxicity mechanisms by introducing mutations into proteins that cause growth inhibition. As a result, we revealed that the presence of specific amino acids or the activity of the protein itself can lead to growth inhibition.

Free Research Field

システム生物学

Academic Significance and Societal Importance of the Research Achievements

本研究は、これまでほとんど分かっていないタンパク質の発現を制約するメカニズムの解明につながり、基礎生物学的な知識の拡張に繋がる。また、ここで得られた知識は、細胞にタンパク質が蓄積することにより生じる疾患、癌や神経変性疾患などの細胞の生理状態の理解と創薬へと繋がるとともに、細胞工学において特定のタンパク質を大量発現させたいときのタンパク質デザインの指針となる。