A chromosome-scale collection of CRISPR mutants in Drosophila

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03246
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 43060:System genome science-related
• Research Institution: Tokyo University of Science (2021-2022); National Institute of Genetics (2020)
• Principal Investigator: Kondo Shu 東京理科大学, 先進工学部生命システム工学科, 准教授 (90408401)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ゲノム編集

Outline of Final Research Achievements

Determining the function of all genes in a genome is one of the major goals of modern biology. Arguably the most informative clue to gene function is the phenotype of loss-of-function mutants. Therefore, it is essential to create mutants for all genes and describe their phenotypes in order to fully understand the genome. In this study, we generated loss-of-function mutants for approximately 2,500 of the 14,000 genes in the Drosophila genes. Of these, only about 25% of the genes were essential for development and survival. Among viable mutants, we found a few that showed abnormalities in higher-order functions such as metabolism and behavior, providing the basis for further detailed analysis.

Free Research Field

遺伝学

Academic Significance and Societal Importance of the Research Achievements

本研究で作成したgRNA系統及びノックアウト系統は、国立遺伝学研究所ショウジョウバエ・ストックセンターより順次公開している（https://shigen.nig.ac.jp/fly/nigfly/）。系統は国内外の研究者が実費のみで購入することが可能であり、既に多くの研究者に活用されている。本研究で作成したノックアウトコレクションは、ショウジョウバエ研究全体を大きく推進する重要なリソースとして、今後長く利用されていくことが期待される。