Ultra-fast observation of cortico-hippocampal functional dynamics

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03295
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
• Research Institution: University of Yamanashi
• Principal Investigator: Shinohara Yoshiaki 山梨大学, 大学院総合研究部, 教授 (10425423)
• Co-Investigator(Kenkyū-buntansha): 大野 伸彦 自治医科大学, 医学部, 教授 (10432155)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 海馬 / 大脳皮質 / 脳波 / カルシウム活動

Outline of Final Research Achievements

We performed simultaneous measurement of hippocampal EEG activity and neocortical calcium activity with transgenic mice. Fortunately, we were able to observe strong activity correlations between the hippocampus and neocortex in both theta condition and slow wave activities. We compared two groups of animals which were reared in socially isolated condition (ISO) and enriched condition (ENR). In both groups, we found a correlation between hippocampal and cortical activity during theta activity as well as sharp wave and ripples (SWR). The former is a novel finding because it is technically difficult due to requirement of high time resolution. Interestingly, we observed stronger correlation between hippocampal EEG activities and neocortical calcium oscillations in ISO animals than ENR groups. After submission, a lot of analyses were demanded by reviewers. Unfortunately, due to lab move and a lot of educational duties, I still need more time to make this paper published.

Free Research Field

神経生理学

Academic Significance and Societal Importance of the Research Achievements

環境依存的な脳機能の修飾は神経科学のみならず、精神医学・神経病理学上も重要な問題であり、豊かな環境飼育がなぜ神経疾患に抵抗性を示すかを解明することは重要である。もともと研究では豊かな環境で脳全般の協調活動が増大するという仮説の下実験を行ったが、それとは正反対の結果を得た。左右海馬では豊かな環境飼育で協調活動が増加するので、環境操作は回路特異的に神経活動を修飾するという結果を示した。