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2022 Fiscal Year Final Research Report

Development of a simultaneous analysis system for multispecies breeding regimes with environmental DNA analysis

Research Project

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Project/Area Number 20H03326
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionKobe University

Principal Investigator

Minamoto Toshifumi  神戸大学, 人間発達環境学研究科, 教授 (50450656)

Co-Investigator(Kenkyū-buntansha) 山中 裕樹  龍谷大学, 先端理工学部, 准教授 (60455227)
Project Period (FY) 2020-04-01 – 2023-03-31
Keywords環境DNA / 環境RNA / 繁殖レジーム / メタバーコーディング
Outline of Final Research Achievements

The study was conducted to obtain information other than distribution, such as reproduction of aquatic organisms, by analyzing nucleic acids in the environment including environmental DNA (eDNA) and environmental RNA (eRNA). We found that it is possible to understand the reproductive regimes of specific species by using nuclear and mitochondrial eDNA, that it is possible to understand the reproductive regimes of multiple species simultaneously using quantitative eDNA metabarcoding, and that it is potentially possible to understand the reproductive regimes from environmental messenger RNA (emRNA) information. These results indicate that we can obtain reproductive information, which is important for the conservation of rare and valuable fishery species, by using environmental biomolecules, eDNA and eRNA.

Free Research Field

分子生態学

Academic Significance and Societal Importance of the Research Achievements

野生生物の繁殖に関わる情報は、生物の保全上貴重である。このような情報を水をすくうだけで得ることができるようになれば、希少種や水産有用魚種の保全のための保護区域の設定などに役立つことが期待される。30 by 30における保護区域の設定においても有用な科学的知見を提供するためのツールとなりうるだろう

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Published: 2024-01-30  

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