The analysis of phase-separated nuclear bodies of NUP-fusion

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03444
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 48040:Medical biochemistry-related
• Research Institution: National Institutes of Biomedical Innovation, Health and Nutrition
• Principal Investigator: Oka Masahiro 国立研究開発法人医薬基盤・健康・栄養研究所, 医薬基盤研究所 創薬デザイン研究センター, プロジェクトリーダー (40432504)
• Co-Investigator(Kenkyū-buntansha): 加藤 明良 大分大学, 医学部, 客員研究員 (00167339)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ヌクレオポリン / 融合遺伝子 / CRM1 / 相分離 / HOX遺伝子

Outline of Final Research Achievements

The purpose of this study was to identify the components of the phase-separated body formed by nucleoporin fusion gene products (nucleoporin fusion proteins) and to determine their function. We found several proteins that interact with nucleoporin fusion proteins. In addition, these interacting proteins and nucleoporin fusion protein co-accumulated on the genome to activate the target genes. Furthermore, analysis of the mutants revealed that phase separation of nucleoporin fusion proteins is important for the accumulation of interacting molecules on target gene regions.

Free Research Field

細胞生物学

Academic Significance and Societal Importance of the Research Achievements

ヌクレオポリン融合遺伝子産物がどのように白血病に寄与しているかについては不明な部分が多い。本成果により、ヌクレオポリン融合遺伝子産物が形成する相分離構造体の構成因子やその重要性の一端が明らかとなった。病理メカニズムの解明や治療法の開発へと繋がる成果と考えられる。