2022 Fiscal Year Final Research Report
Involvement of abnormal glycosylation in the pathogenesis of Parkinson's disease
Project/Area Number |
20H03584
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 52010:General internal medicine-related
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Research Institution | Department of Clinical Research, National Hospital Organization Tokushima National Hospital |
Principal Investigator |
Kurota Yukiko 独立行政法人国立病院機構徳島病院(臨床研究部), その他部局等, 研究員(移行) (70398014)
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Co-Investigator(Kenkyū-buntansha) |
三ツ井 貴夫 独立行政法人国立病院機構徳島病院(臨床研究部), その他部局等, その他 (80294726)
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Project Period (FY) |
2020-04-01 – 2023-03-31
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Keywords | パーキンソン病 |
Outline of Final Research Achievements |
We have found that Parkin is intracellularly modified with O-GlcNAc (O-GlcNAcylation) and localized to mitochondria. When the deglycosylating enzymes O-glycosidase and Sialidase A were added simultaneously to the glycosylated parkin, there was a shift in the height of the bands of the non-glycosylated Parkin. Monosaccharide analysis suggested that Parkin was O-glycosylated.Glycoblotting analysis showed that (HexNAc)1 was the major component, and the Click-iT reaction also confirmed O-GlcNAc modification.
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Free Research Field |
分子生物学
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Academic Significance and Societal Importance of the Research Achievements |
本研究で得られた知見より、哺乳動物細胞内でパーキンの一部はO-GlcNAcを含むO型糖鎖が付加されていることは確実である。我々は本研究においてO-GlcNAc 修飾機構の異常がパーキンソン病の発症要因になりうるか否かを、家族性パーキンソン病である PARK2 において、さらには孤発性パーキンソン病において明らかにするために計画された。
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