Early diagnosis of pancreatic cancer based on diversity of field defect and molecular genetic approach

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03655
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 53010:Gastroenterology-related
• Research Institution: Asahikawa Medical College
• Principal Investigator: Mizukami Yusuke 旭川医科大学, 医学部, 教授 (30400089)
• Co-Investigator(Kenkyū-buntansha): 小野 裕介 医療法人徳洲会札幌東徳洲会病院医学研究所, ゲノム診断研究部, 部門長 (40742648) ; 唐崎 秀則 医療法人徳洲会札幌東徳洲会病院医学研究所, がん生物研究部, 客員研究員 (50374806) ; 谷上 賢瑞 東京大学, アイソトープ総合センター, 特任准教授 (90648627)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 膵癌 / IPMN / クローン進化 / 分子サブタイプ

Outline of Final Research Achievements

Our goal was to create detailed maps of multiple lesions found in surgically removed pancreatic specimens, with a focus on pancreatic cancer related to IPMN. We discovered a variety of genetic mutations in precursor lesions located in the pancreas. We also extracted a portion of the tumor cells from the removed material and conducted RNA-Seq analysis and long-read sequencing using nanopores. With the results, we attempted to assess the clonal affinity by analyzing the genetic abnormalities accumulation pattern from low-grade to high-grade lesions, and we reconstructed the evolution timeline within the lesion. Moreover, we are analyzing the same region's spatial transcriptome and comparing it with the mutation distribution. We are currently working on the manuscript, and the data analysis is underway.

Free Research Field

消化器内科、腫瘍生物学

Academic Significance and Societal Importance of the Research Achievements

膵発癌素地の詳細な分子情報を基盤とするマルチバイオ検出系への展開は、早期診断のみならず、個体の発癌危険性を予測するうえで大きな意義がある。また膵癌の初期発生に関わる起源細胞を明らかにすることは、新規治療標的の特定はもとより、究極的には予防医学への布石となる。