Induction and analysis of regulatory T cells by ultraviolet irradiation - Development of liquid biopsy for skin lesions

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H03703
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 53050:Dermatology-related
• Research Institution: Nagoya City University
• Principal Investigator: Morita Akimichi 名古屋市立大学, 医薬学総合研究院(医学), 教授 (30264732)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 紫外線 / 制御性T細胞 / リキッドバイオプシー

Outline of Final Research Achievements

A lesional blood liquid biopsy of cutaneous T-cell lymphoma (CTCL) was performed. CD4+CD45RO+ cells were significantly more abundant in leional blood. mSWAT was inversely correlated with CD8+CD45RO+ cells in lesions. chemokines that induce malignant T cells, such as CCL17 and CCL22, were higher in lesional blood, RNA-seq showed high expression of CTCL tumor cell-specific genes, and TCR repertoire analysis revealed a polarized T-cell receptor. Lesional blood contained skin disease-specific cells and proteins, establishing a technique for lesional blood liquid biopsy. The study of regulatory T cell induction wavelengths revealed that the expression of IL-10 and CCL22 increased 2- to 3-fold in the UVC-UVB wavelengths, and the "Tregness" of CD4-positive T cells was increased by UV irradiation at 260 nm, 280 nm, and 300 nm.

Free Research Field

皮膚科学

Academic Significance and Societal Importance of the Research Achievements

本研究によって、皮膚疾患の病変の皮疹血から、疾患特異的な細胞やタンパク質を十分に解析することができ、臨床レベルでも他の炎症疾患の診断に役立つ可能性があることが示唆された。臨床レベルにはつなげることはできていないが、制御性Ｔ細胞が、UVC～UVBの短い波長で誘導されることもあきらかにし、今後、制御性Ｔ細胞の誘導が最適となるような光線療法の開発が、リキッドバイオプシーによって可能になると思われる。