2022 Fiscal Year Final Research Report

Therapeutic effect of adipose-derived stromal vascular fraction on osteoarthritic chondrocytes

Research Project

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Project/Area Number	20H03804
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 56020:Orthopedics-related
Research Institution	Kobe University
Principal Investigator	Kuroda Ryosuke 神戸大学, 医学研究科, 教授 (80379362)
Co-Investigator(Kenkyū-buntansha)	松下雄彦神戸大学, 医学研究科, 特命准教授 (40467650) 松本知之神戸大学, 医学研究科, 准教授 (50546588) 岩畔英樹獨協医科大学, 医学部, 非常勤講師 (60366002)
Project Period (FY)	2020-04-01 – 2023-03-31
Keywords	脂肪幹細胞
Outline of Final Research Achievements	Basic study aimed to investigate the therapeutic effect of heterogeneous cells in adipose-derived stromal vascular fraction (SVF) on chondrocytes. Chondrocytes (1 × 105 cells) were seeded in culture plates, and co-cultured with SVF (1× 105 cells) or ADSC (1 × 104 cells) on cell culture inserts. The findings including RT-PCR, immunohistochemical staining of macrophages, and western blotting of smad2/3 pathway suggested that the paracrine effect of heterogeneous cells including anti-inflammatory macrophages in SVF partly supports the regeneration of chondrocytes through TGF-β-induced Smad2/3 phosphorylation. Clinical study aimed to compare the clinical and radiological outcomes between the single and twice SVF cell injection for knee osteoarthritis (OA) patients. . Based on clinical score and MRI findings concluded that the twice injections could be required for satisfactory treatment of patients with severe varus knee alignment.
Free Research Field	再生医療
Academic Significance and Societal Importance of the Research Achievements	脂肪幹細胞の変形性膝関節症治療に対する有用性は報告されているが、その機序は不明な点も多かった。脂肪幹細胞に含まれるM2マクロファージがSmad2/3リン酸化を介して関与する、という本研究で解明された機序は、今後臨床における治療法の向上に寄与するものであり学術的意義が高いものと考える。また臨床試験における高度変形膝を有する患者には脂肪幹細胞1回注射よりも2回注射が有効である結果をうけて、実際の個々の患者における治療の適応がより明確になる、という点で社会的意義が高いと考える。