Discovery and development of new pathologies involving fungal allergy in intractable sinusitis

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H03832
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Akita University
• Principal Investigator: Yamada Takechiyo 秋田大学, 医学系研究科, 教授 (70283182)
• Co-Investigator(Kenkyū-buntansha): 海老原 敬 秋田大学, 医学系研究科, 教授 (20374407) ; 植木 重治 秋田大学, 医学系研究科, 教授 (60361234)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: ILC2 / Asp f 1 / IgE / IL-4 / Galectin-10 / TIGIT

Outline of Final Research Achievements

Nasal polyp tissues were collected from 103 patients with CRSwNP during surgery and homogenized. Tissue eosinophil counts and Asp f1, Aspergillus-specific IgE, and related molecules were measured in nasal polyps. Eighteen samples (15.9%) tested positive for Asp f 1 and were divided into two groups based on Asp f 1 levels. Asp f 1 levels positively correlated with Aspergillus-specific IgE levels (r =0.68, p <0.01). Aspergillus-specific IgE, IgE, and IgG levels in nasal polyps were significantly higher in the high Asp f 1 group than in the low Asp f 1 group although no significant differences were observed in IgM levels between these groups. Furthermore, Asp f 1 levels positively correlated with tissue IL-4 levels (r=0.85, p<0.0005) or tissue galectin-10 levels (r=0.54, p<0.01).

Free Research Field

耳鼻咽喉科頭頸部外科

Academic Significance and Societal Importance of the Research Achievements

ヒト鼻茸組織の中Asp f 1 抗原を定量し, Aspergillus specific IgE , IL-4 レベル , Galectin-10レベル (rs = 0.54) (p＜ 0.01)の相関を示した（Allergy. 2022;77:3154-3156）。また, 真菌アレルギーなどで遷延化する強いアレルギー炎症や鼻茸組織には自然リンパ球type 2 (ILC2s) が豊富に存在し、TGITやCD155が慢性アレルギーの新しいバイオマーカー, 治療標的となる可能性が示唆された(J Exp Med. 2023;220:e20222005)。それぞれ，世界初めての報告である。