Searching for optimal conditions for autogenous bone grafting focusing on bone cell network reconstruction and bone quality in grafted bone

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H03876
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Niigata University
• Principal Investigator: Uoshima Katsumi 新潟大学, 医歯学系, 教授 (50213400)
• Co-Investigator(Kenkyū-buntansha): 加来 賢 新潟大学, 医歯学系, 准教授 (30547542) ; 長澤 麻沙子 新潟大学, 医歯学系, 助教 (40612239) ; 秋葉 陽介 新潟大学, 医歯学総合病院, 講師 (70547512)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 骨移植 / 骨細管 / 骨吸収 / ネットワーク

Outline of Final Research Achievements

Through trial and error, we found the optimal protocol for bone tissue transparency (Cubic method). In addition, we used two-photon microscopy and silver staining to observe the bone canalicular network in the grafted bone after bone grafting. Two weeks after grafting, bone formation was observed between the grafted bone and the parent bone, and regeneration of bone canals, which was not observed one week later, was observed. In the future, we will set up a long-term experimental period and be able to observe the relationship between the disruption and regeneration of the bone canalicular network in the entire grafted bone and bone resorption from around the grafted bone.

Free Research Field

補綴歯科学

Academic Significance and Societal Importance of the Research Achievements

これまでに報告されていない骨組織の透明化によって、切片ではない骨標本の骨細管を立体的に観察できるようになった。また、骨移植に伴う骨細管ネットワークの寸断と再生に関する観察を行うことができ、今後の長期実験期間の設定によって、当初の目的である移植骨の吸収と骨細管ネットワークの再生の関係性が見出せる端緒となった。