Repository of sleep bruxism and elucidation of its mechanism based on disease-specific iPS cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 20H03882
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 57050:Prosthodontics-related
• Research Institution: Showa University
• Principal Investigator: Baba Kazuyoshi 昭和大学, 歯学部, 教授 (80251536)
• Co-Investigator(Kenkyū-buntansha): 赤松 和土 順天堂大学, 大学院医学研究科, 教授 (60338184) ; 西山 暁 東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (40359675) ; 美島 健二 昭和大学, 歯学部, 教授 (50275343) ; 中村 史朗 昭和大学, 歯学部, 教授 (60384187) ; 高場 雅之 昭和大学, 歯学部, 講師 (30384192) ; 井上 富雄 昭和大学, 歯学部, 教授 (70184760)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Keywords: 睡眠時ブラキシズム / iPS細胞

Outline of Final Research Achievements

The present study aimed to establish a repository of sleep bruxism (SB) with a goal of analysing functional differences in neurons due to single nucleotide polymorphisms associated with sleep bruxism (SB) found in a previous study, and to detect and phenotypically classify functional abnormalities across neurons derived from SB patients at the genomic level. Clinical examination findings and muscle activity levels during sleep were recorded from the subjects and entered into a repository as a database. In addition, iPS cells from SB patients and controls were established and induced into neurons. Neurons were used to search for differentially expressed genes by transcriptome analysis, and the results suggested that there were systematic differences in gene expression patterns between the SB patient and control groups.

Free Research Field

補綴歯科学

Academic Significance and Societal Importance of the Research Achievements

睡眠時ブラキシズム(睡眠中の歯ぎしり)は様々な歯科的問題の原因となり得るが、メカニズムはまだ不明な点も多い。この研究によって構築された睡眠時ブラキシズムのリポジトリを発展させ、さらにiPS細胞由来神経細胞での解析がより詳細に行われることで、発症メカニズムの解明が進むことが期待でき、この成果は、個々人の病態に応じて歯科医療を提供する、オーダーメイド医療に寄与することができると考えられる。