2022 Fiscal Year Final Research Report
Establishment of new oral biofilm formation control system targeting membrane proteins
| Project/Area Number |
20H03897
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 57070:Developmental dentistry-related
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| Research Institution | Okayama University |
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Principal Investigator |
Nakano Michiyo (松本道代) 岡山大学, 医歯薬学域, 教授 (30359848)
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| Co-Investigator(Kenkyū-buntansha) |
仲 周平 岡山大学, 大学病院, 講師 (10589774)
稲葉 裕明 岡山大学, 医歯薬学域, 准教授 (70359850)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | Streptococcus mutans / バイオフィルム / 膜タンパク / ABC膜輸送体 |
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| Outline of Final Research Achievements |
Streptococcus mutans, reported to be associated with dental caries induction, also forms biofilm. ATP-binding cassette (ABC) transporters exist in cell membranes, and function to export and import molecules. In the present study, functional analyses of the SMU1519 gene, predicted to be a zinc transporter, regarding its relationship to biofilm formation by S. mutans were performed. S. mutans MT8148 (serotype c) and an isogenic mutant strain with inactivation of the SMU1519 gene, termed ΔSMU1519, were used. The growth rate of ΔSMU1519 was slightly lower than that of MT8148, though in the presence of ZnSO4, there was no difference between them. Furthermore, while the biomass of biofilms formed by ΔSMU1519 was significantly lower than that of MT8148 biofilms, the difference between them was even greater in the presence of ZnSO4.
These results suggest that SMU1519 contributes to zinc transport by S. mutans, which is related to biofilm formation by the organism.
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| Free Research Field |
小児歯科学
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| Academic Significance and Societal Importance of the Research Achievements |
Streptococcus mutans の保有するABC膜輸送体はクオラムセンシングの主要タンパクである膜タンパクの一つであり、菌の生存に必要な物質の輸送だけでなく、バイオフィルム形成能に関与する可能性が示唆されている。クオラムセンシングによってバイフォイルム形成は制御されているため、ABC膜輸送体の機能を明らかにすることはバイフォイルム形成制御メカニズムの解明につながる可能性が高い。本研究で同定したABC膜輸送体は亜鉛の輸送体として機能し、バイオフィルム形成に関与していた。この結果は、バイオフィルム形成を抑制物質を開発する上で、ABC膜輸送体が新たなターゲットとなる可能性が示された。
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