2022 Fiscal Year Final Research Report
Elucidation of mechanism of prooxidant effect on cancer cells by polyphenols
| Project/Area Number |
20H03926
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 58020:Hygiene and public health-related: including laboratory approach
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
斎藤 健 北海道大学, 保健科学研究院, 客員研究員 (40153811)
佐藤 伸 青森県立保健大学, 健康科学部, 教授 (40310099)
向井 友花 神奈川県立保健福祉大学, 保健福祉学部, 教授 (60331211)
藏崎 正明 北海道大学, 地球環境科学研究院, 客員研究員 (80161727)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | ポリフェノール / 抗酸化作用 / プロオキシダント作用 / 癌細胞 / アポトーシス |
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| Outline of Final Research Achievements |
HepG2 and Caco-2 cells derived from human cancer cells were exposed to various concentrations of resveratrol or epigallocatechin gallate. As results, cell viavility of the human cancer cells exposed to the polyphenol was increased in a dose-dependent manner, and the oxidative state in the cells was enhanced.
Analyses by Western blotting and other biochemical and cell biological methods confirmed that exposure to polyphenols in the human cancer cells induced apoptosis via autophagy. On the other hand, when human normal hepatocytes, HUCPG cells, were exposed to resveratrol, no significant changes in apoptotic factors were observed, and there was no sign of apoptosis induction in the cells.
From these results, it was clarified that apoptosis was induced only in cancer cells when exposed to polyphenols.
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| Free Research Field |
衛生学
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| Academic Significance and Societal Importance of the Research Achievements |
世界的に年々増加している様々な癌の治療において化学療法は重要な選択肢の一つであるが、しばしばその重篤な副作用が大きな問題となっている。安全で副作用の少ない抗癌剤あるいは抗癌補助剤の開発は癌治療戦略において緊喫の学術意義のある課題である。本研究で示された植物成分の一種であり毒性が少ないポリフェノール類が癌細胞にアポトーシスを誘導するという実験結果は将来の安全な抗癌補助剤の開発のための重要なステップになることが大いに期待される。
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