Multi-bioimaging of target molecules controlling skeletal muscle fiber adaptation

2022 Fiscal Year Final Research Report

• Project/Area Number: 20H04074
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 59020:Sports sciences-related
• Research Institution: The University of Electro-Communications
• Principal Investigator: Kano Yutaka 電気通信大学, 大学院情報理工学研究科, 教授 (90293133)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Keywords: 骨格筋 / カルシウムイオン / 活性酸素種 / バイオイメージング

Outline of Final Research Achievements

The aim of this study was to evaluate the spatiotemporal patterns of intracellular calcium ion concentration ([Ca2+]i) and hydrogen peroxide concentration ([H2O2]i) involved in skeletal muscle adaptation and to elucidate their regulatory mechanisms. The main findings are as follows: 1) In the eccentric contraction mode of skeletal muscle, the [Ca2+]i variation pattern is dependent on muscle fiber extension; 2) Post-exercise [Ca2+]i dynamics is regulated by sarcoplasmic reanodine receptors, which are involved in the induction of muscle damage; 3) Changes in [H2O2]i associated with muscle contraction are small, indicating that [H2O2]i is not the main factor inducing Ca2+ accumulation during exercise.

Free Research Field

運動生理学

Academic Significance and Societal Importance of the Research Achievements

本研究は，筋肉量やミトコンドリア量をコントロールする細胞内のスイッチ機構の存在がカルシウムイオンと活性酸素種のダイナミクス（細胞質中の空間的・時間的な濃度変化の連続）と関係することを明らかにした．このスイッチ機構の解明は，アスリートをはじめ，高齢者に対する筋肉量やミトコンドリア量をコントロールする運動プログラムの開発に応用できるため，社会的にも大きな意義を有している．